Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth4.fastp-trim.202003062011_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth4.fastp-trim.202003062011_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 6120509 Mapping efficiency: 61.2% Sequence pairs with no alignments under any condition: 2816684 Sequence pairs did not map uniquely: 1062807 Sequence pairs which were discarded because genomic sequence could not be extracted: 64495 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1477309 ((converted) top strand) GA/CT/CT: 1570562 (complementary to (converted) top strand) GA/CT/GA: 1544153 (complementary to (converted) bottom strand) CT/GA/GA: 1463990 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 275557099 Total methylated C's in CpG context: 7255070 Total methylated C's in CHG context: 673169 Total methylated C's in CHH context: 1611275 Total methylated C's in Unknown context: 27957 Total unmethylated C's in CpG context: 54766881 Total unmethylated C's in CHG context: 53968417 Total unmethylated C's in CHH context: 157282287 Total unmethylated C's in Unknown context: 483629 C methylated in CpG context: 11.7% C methylated in CHG context: 1.2% C methylated in CHH context: 1.0% C methylated in unknown context (CN or CHN): 5.5% Bismark completed in 0d 0h 45m 51s