Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth3.fastp-trim.202003064031_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth3.fastp-trim.202003064031_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 4793355 Mapping efficiency: 47.9% Sequence pairs with no alignments under any condition: 4913337 Sequence pairs did not map uniquely: 293308 Sequence pairs which were discarded because genomic sequence could not be extracted: 21427 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1130703 ((converted) top strand) GA/CT/CT: 1247528 (complementary to (converted) top strand) GA/CT/GA: 1257554 (complementary to (converted) bottom strand) CT/GA/GA: 1136143 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 211013813 Total methylated C's in CpG context: 1116568 Total methylated C's in CHG context: 480250 Total methylated C's in CHH context: 1694980 Total methylated C's in Unknown context: 129866 Total unmethylated C's in CpG context: 31759707 Total unmethylated C's in CHG context: 38128524 Total unmethylated C's in CHH context: 137833784 Total unmethylated C's in Unknown context: 740254 C methylated in CpG context: 3.4% C methylated in CHG context: 1.2% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 14.9% Bismark completed in 0d 0h 40m 1s