Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth9.fastp-trim.202003062409_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth9.fastp-trim.202003062409_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 32972 Mapping efficiency: 33.0% Sequence pairs with no alignments under any condition: 64506 Sequence pairs did not map uniquely: 2522 Sequence pairs which were discarded because genomic sequence could not be extracted: 210 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8027 ((converted) top strand) GA/CT/CT: 8397 (complementary to (converted) top strand) GA/CT/GA: 8259 (complementary to (converted) bottom strand) CT/GA/GA: 8079 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1437369 Total methylated C's in CpG context: 41310 Total methylated C's in CHG context: 4480 Total methylated C's in CHH context: 15680 Total methylated C's in Unknown context: 1252 Total unmethylated C's in CpG context: 164156 Total unmethylated C's in CHG context: 265027 Total unmethylated C's in CHH context: 946716 Total unmethylated C's in Unknown context: 5534 C methylated in CpG context: 20.1% C methylated in CHG context: 1.7% C methylated in CHH context: 1.6% C methylated in unknown context (CN or CHN): 18.4% Bismark completed in 0d 0h 1m 43s