Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth7.fastp-trim.202003065422_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth7.fastp-trim.202003065422_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 32152 Mapping efficiency: 32.2% Sequence pairs with no alignments under any condition: 65112 Sequence pairs did not map uniquely: 2736 Sequence pairs which were discarded because genomic sequence could not be extracted: 227 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7630 ((converted) top strand) GA/CT/CT: 8090 (complementary to (converted) top strand) GA/CT/GA: 8317 (complementary to (converted) bottom strand) CT/GA/GA: 7888 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1437739 Total methylated C's in CpG context: 54423 Total methylated C's in CHG context: 4275 Total methylated C's in CHH context: 15249 Total methylated C's in Unknown context: 1214 Total unmethylated C's in CpG context: 158413 Total unmethylated C's in CHG context: 265111 Total unmethylated C's in CHH context: 940268 Total unmethylated C's in Unknown context: 5545 C methylated in CpG context: 25.6% C methylated in CHG context: 1.6% C methylated in CHH context: 1.6% C methylated in unknown context (CN or CHN): 18.0% Bismark completed in 0d 0h 1m 43s