Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth6.fastp-trim.202003062949_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth6.fastp-trim.202003062949_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 61641 Mapping efficiency: 61.6% Sequence pairs with no alignments under any condition: 27989 Sequence pairs did not map uniquely: 10370 Sequence pairs which were discarded because genomic sequence could not be extracted: 658 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 14756 ((converted) top strand) GA/CT/CT: 15877 (complementary to (converted) top strand) GA/CT/GA: 15751 (complementary to (converted) bottom strand) CT/GA/GA: 14599 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2788835 Total methylated C's in CpG context: 71519 Total methylated C's in CHG context: 6158 Total methylated C's in CHH context: 15028 Total methylated C's in Unknown context: 283 Total unmethylated C's in CpG context: 548574 Total unmethylated C's in CHG context: 542178 Total unmethylated C's in CHH context: 1605378 Total unmethylated C's in Unknown context: 4909 C methylated in CpG context: 11.5% C methylated in CHG context: 1.1% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 5.5% Bismark completed in 0d 0h 1m 53s