Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth12.fastp-trim.202003060645_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth12.fastp-trim.202003060645_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 2654 Mapping efficiency: 26.5% Sequence pairs with no alignments under any condition: 6435 Sequence pairs did not map uniquely: 911 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 578 ((converted) top strand) GA/CT/CT: 742 (complementary to (converted) top strand) GA/CT/GA: 694 (complementary to (converted) bottom strand) CT/GA/GA: 640 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 128204 Total methylated C's in CpG context: 2858 Total methylated C's in CHG context: 247 Total methylated C's in CHH context: 686 Total methylated C's in Unknown context: 10 Total unmethylated C's in CpG context: 18312 Total unmethylated C's in CHG context: 22560 Total unmethylated C's in CHH context: 83541 Total unmethylated C's in Unknown context: 97 C methylated in CpG context: 13.5% C methylated in CHG context: 1.1% C methylated in CHH context: 0.8% C methylated in unknown context (CN or CHN): 9.3% Bismark completed in 0d 0h 0m 21s