Bismark report for: /gscratch/scrubbed/sr320/froger-raw/00_fastq/Meth14_R1_001.fastq.gz and /gscratch/scrubbed/sr320/froger-raw/00_fastq/Meth14_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome_02/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 2134 Mapping efficiency: 21.3% Sequence pairs with no alignments under any condition: 7604 Sequence pairs did not map uniquely: 262 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 563 ((converted) top strand) GA/CT/CT: 508 (complementary to (converted) top strand) GA/CT/GA: 502 (complementary to (converted) bottom strand) CT/GA/GA: 561 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 129470 Total methylated C's in CpG context: 3274 Total methylated C's in CHG context: 208 Total methylated C's in CHH context: 555 Total methylated C's in Unknown context: 22 Total unmethylated C's in CpG context: 25305 Total unmethylated C's in CHG context: 22226 Total unmethylated C's in CHH context: 77902 Total unmethylated C's in Unknown context: 80 C methylated in CpG context: 11.5% C methylated in CHG context: 0.9% C methylated in CHH context: 0.7% C methylated in unknown context (CN or CHN): 21.6% Bismark completed in 0d 0h 0m 26s