Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth5.fastp-trim.202003051832_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth5.fastp-trim.202003051832_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 80 Mapping efficiency: 0.8% Sequence pairs with no alignments under any condition: 9777 Sequence pairs did not map uniquely: 143 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10 ((converted) top strand) GA/CT/CT: 22 (complementary to (converted) top strand) GA/CT/GA: 25 (complementary to (converted) bottom strand) CT/GA/GA: 23 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2238 Total methylated C's in CpG context: 65 Total methylated C's in CHG context: 18 Total methylated C's in CHH context: 211 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 371 Total unmethylated C's in CHG context: 369 Total unmethylated C's in CHH context: 1204 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 14.9% C methylated in CHG context: 4.7% C methylated in CHH context: 14.9% Can't determine percentage of methylated Cs in unknown context (CN or CHN) if value was 0 Bismark completed in 0d 0h 0m 21s