Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth15.fastp-trim.202003050057_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth15.fastp-trim.202003050057_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 4676 Mapping efficiency: 46.8% Sequence pairs with no alignments under any condition: 2459 Sequence pairs did not map uniquely: 2865 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1100 ((converted) top strand) GA/CT/CT: 1246 (complementary to (converted) top strand) GA/CT/GA: 1223 (complementary to (converted) bottom strand) CT/GA/GA: 1107 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 221045 Total methylated C's in CpG context: 6405 Total methylated C's in CHG context: 448 Total methylated C's in CHH context: 1126 Total methylated C's in Unknown context: 2 Total unmethylated C's in CpG context: 41939 Total unmethylated C's in CHG context: 41059 Total unmethylated C's in CHH context: 130068 Total unmethylated C's in Unknown context: 87 C methylated in CpG context: 13.2% C methylated in CHG context: 1.1% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 2.2% Bismark completed in 0d 0h 0m 21s