Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth16.fastp-trim.202003062412_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth16.fastp-trim.202003062412_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 71022035 Number of paired-end alignments with a unique best hit: 1575120 Mapping efficiency: 2.2% Sequence pairs with no alignments under any condition: 68790096 Sequence pairs did not map uniquely: 656819 Sequence pairs which were discarded because genomic sequence could not be extracted: 30 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 388193 ((converted) top strand) GA/CT/CT: 398871 (complementary to (converted) top strand) GA/CT/GA: 398181 (complementary to (converted) bottom strand) CT/GA/GA: 389845 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 73432804 Total methylated C's in CpG context: 1918726 Total methylated C's in CHG context: 205584 Total methylated C's in CHH context: 693863 Total methylated C's in Unknown context: 71104 Total unmethylated C's in CpG context: 10461217 Total unmethylated C's in CHG context: 12864401 Total unmethylated C's in CHH context: 47289013 Total unmethylated C's in Unknown context: 242835 C methylated in CpG context: 15.5% C methylated in CHG context: 1.6% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 22.6% Bismark completed in 0d 0h 38m 1s