Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth18.fastp-trim.202003065117_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth18.fastp-trim.202003065117_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 2380508 Mapping efficiency: 23.8% Sequence pairs with no alignments under any condition: 6684636 Sequence pairs did not map uniquely: 934856 Sequence pairs which were discarded because genomic sequence could not be extracted: 39 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 583699 ((converted) top strand) GA/CT/CT: 605059 (complementary to (converted) top strand) GA/CT/GA: 605925 (complementary to (converted) bottom strand) CT/GA/GA: 585786 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 111356510 Total methylated C's in CpG context: 2584114 Total methylated C's in CHG context: 292304 Total methylated C's in CHH context: 1011000 Total methylated C's in Unknown context: 98611 Total unmethylated C's in CpG context: 16407834 Total unmethylated C's in CHG context: 19535993 Total unmethylated C's in CHH context: 71525265 Total unmethylated C's in Unknown context: 349797 C methylated in CpG context: 13.6% C methylated in CHG context: 1.5% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 22.0% Bismark completed in 0d 0h 23m 55s