Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth13.fastp-trim.202003062040_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth13.fastp-trim.202003062040_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 5622812 Mapping efficiency: 56.2% Sequence pairs with no alignments under any condition: 1442903 Sequence pairs did not map uniquely: 2934285 Sequence pairs which were discarded because genomic sequence could not be extracted: 48 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1384531 ((converted) top strand) GA/CT/CT: 1429215 (complementary to (converted) top strand) GA/CT/GA: 1428844 (complementary to (converted) bottom strand) CT/GA/GA: 1380174 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 277384802 Total methylated C's in CpG context: 7369536 Total methylated C's in CHG context: 398813 Total methylated C's in CHH context: 1024709 Total methylated C's in Unknown context: 6501 Total unmethylated C's in CpG context: 51494487 Total unmethylated C's in CHG context: 50945862 Total unmethylated C's in CHH context: 166151395 Total unmethylated C's in Unknown context: 292362 C methylated in CpG context: 12.5% C methylated in CHG context: 0.8% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 2.2% Bismark completed in 0d 0h 44m 56s