Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth12.fastp-trim.202003060645_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth12.fastp-trim.202003060645_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 4108852 Mapping efficiency: 41.1% Sequence pairs with no alignments under any condition: 4447619 Sequence pairs did not map uniquely: 1443529 Sequence pairs which were discarded because genomic sequence could not be extracted: 45 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 959725 ((converted) top strand) GA/CT/CT: 1094950 (complementary to (converted) top strand) GA/CT/GA: 1095341 (complementary to (converted) bottom strand) CT/GA/GA: 958791 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 194938111 Total methylated C's in CpG context: 4269569 Total methylated C's in CHG context: 488829 Total methylated C's in CHH context: 1611411 Total methylated C's in Unknown context: 149357 Total unmethylated C's in CpG context: 28243352 Total unmethylated C's in CHG context: 34169768 Total unmethylated C's in CHH context: 126155182 Total unmethylated C's in Unknown context: 624496 C methylated in CpG context: 13.1% C methylated in CHG context: 1.4% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 19.3% Bismark completed in 0d 0h 35m 53s