Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/froger/trim/Mc/Meth15.fastp-trim.202003065503_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth15.fastp-trim.202003065503_R2_001.fastq.gz

Data processed at 17:15 on 2020-03-09

Alignment Stats

Sequence pairs analysed in total 100000000
Paired-end alignments with a unique best hit 53148550
Pairs without alignments under any condition 15564204
Pairs that did not map uniquely 31287246
Genomic sequence context not extractable (edges of chromosomes) 367

Cytosine Methylation

Total C's analysed 2543980988
Methylated C's in CpG context 72241382
Methylated C's in CHG context 5440664
Methylated C's in CHH context 14488776
Methylated C's in Unknown context 81069
Unmethylated C's in CpG context 475981494
Unmethylated C's in CHG context 469693006
Unmethylated C's in CHH context 1506135666
Unmethylated C's in Unknown context 2701046
Percentage methylation (CpG context) 13.2%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 12843923 original top strand
CTOT 13723742 complementary to original top strand
CTOB 13736475 complementary to original bottom strand
OB 12844043 original bottom strand

Cytosine Methylation after Extraction

Total C's analysed 1655865988
Methylated C's in CpG context 51785560
Methylated C's in CHG context 3610280
Methylated C's in CHH context 9230839
Unmethylated C's in CpG context 311383529
Unmethylated C's in CHG context 302848168
Unmethylated C's in CHH context 977007612
Percentage methylation (CpG context) 14.3%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 0.9%

M-Bias Plot