Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/froger/trim/Mc/Meth14.fastp-trim.202003064415_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth14.fastp-trim.202003064415_R2_001.fastq.gz

Data processed at 17:15 on 2020-03-09

Alignment Stats

Sequence pairs analysed in total 67906601
Paired-end alignments with a unique best hit 36854904
Pairs without alignments under any condition 8823555
Pairs that did not map uniquely 22228142
Genomic sequence context not extractable (edges of chromosomes) 143

Cytosine Methylation

Total C's analysed 1734079017
Methylated C's in CpG context 48772737
Methylated C's in CHG context 2486241
Methylated C's in CHH context 6151420
Methylated C's in Unknown context 42540
Unmethylated C's in CpG context 334572229
Unmethylated C's in CHG context 326292470
Unmethylated C's in CHH context 1015803920
Unmethylated C's in Unknown context 1747598
Percentage methylation (CpG context) 12.7%
Percentage methylation (CHG context) 0.8%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 10153454 original top strand
CTOT 8271163 complementary to original top strand
CTOB 8271951 complementary to original bottom strand
OB 10158193 original bottom strand

Cytosine Methylation after Extraction

Total C's analysed 1087370081
Methylated C's in CpG context 35399081
Methylated C's in CHG context 1652071
Methylated C's in CHH context 3835858
Unmethylated C's in CpG context 211028642
Unmethylated C's in CHG context 202870147
Unmethylated C's in CHH context 632584282
Percentage methylation (CpG context) 14.4%
Percentage methylation (CHG context) 0.8%
Percentage methylation (CHH context) 0.6%

M-Bias Plot