Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/froger/trim/Mc/Meth13.fastp-trim.202003062040_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth13.fastp-trim.202003062040_R2_001.fastq.gz

Data processed at 17:15 on 2020-03-09

Alignment Stats

Sequence pairs analysed in total 98707822
Paired-end alignments with a unique best hit 55417759
Pairs without alignments under any condition 14281343
Pairs that did not map uniquely 29008720
Genomic sequence context not extractable (edges of chromosomes) 415

Cytosine Methylation

Total C's analysed 2730281770
Methylated C's in CpG context 72681585
Methylated C's in CHG context 3919017
Methylated C's in CHH context 10017051
Methylated C's in Unknown context 64862
Unmethylated C's in CpG context 506801931
Unmethylated C's in CHG context 501441629
Unmethylated C's in CHH context 1635420557
Unmethylated C's in Unknown context 2886548
Percentage methylation (CpG context) 12.5%
Percentage methylation (CHG context) 0.8%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 13664437 original top strand
CTOT 14080291 complementary to original top strand
CTOB 14058784 complementary to original bottom strand
OB 13613832 original bottom strand

Cytosine Methylation after Extraction

Total C's analysed 1849249186
Methylated C's in CpG context 53368843
Methylated C's in CHG context 2733403
Methylated C's in CHH context 6708214
Unmethylated C's in CpG context 343947604
Unmethylated C's in CHG context 336608906
Unmethylated C's in CHH context 1105882216
Percentage methylation (CpG context) 13.4%
Percentage methylation (CHG context) 0.8%
Percentage methylation (CHH context) 0.6%

M-Bias Plot