Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/froger/trim/Mc/Meth11.fastp-trim.202003065734_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth11.fastp-trim.202003065734_R2_001.fastq.gz

Data processed at 10:51 on 2020-03-09


Alignment Stats

Sequence pairs analysed in total 55809314
Paired-end alignments with a unique best hit 1753832
Pairs without alignments under any condition 53380684
Pairs that did not map uniquely 674798
Genomic sequence context not extractable (edges of chromosomes) 29

Cytosine Methylation

Total C's analysed 81765934
Methylated C's in CpG context 1781666
Methylated C's in CHG context 188076
Methylated C's in CHH context 646329
Methylated C's in Unknown context 47916
Unmethylated C's in CpG context 12041967
Unmethylated C's in CHG context 14571661
Unmethylated C's in CHH context 52536235
Unmethylated C's in Unknown context 237161
Percentage methylation (CpG context) 12.9%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 405991 original top strand
CTOT 470482 complementary to original top strand
CTOB 471496 complementary to original bottom strand
OB 405834 original bottom strand

Deduplication

Alignments analysed 1753803
Unique alignments 1210459
Duplicates removed 543344
Duplicated alignments were found at 372282 different positions

Cytosine Methylation after Extraction

Total C's analysed 37598881
Methylated C's in CpG context 823615
Methylated C's in CHG context 86740
Methylated C's in CHH context 301751
Unmethylated C's in CpG context 5515832
Unmethylated C's in CHG context 6663115
Unmethylated C's in CHH context 24207828
Percentage methylation (CpG context) 13.0%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 1.2%

M-Bias Plot