Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/froger/trim/Mc/Meth10.fastp-trim.202003063900_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth10.fastp-trim.202003063900_R2_001.fastq.gz

Data processed at 10:51 on 2020-03-09


Alignment Stats

Sequence pairs analysed in total 81884259
Paired-end alignments with a unique best hit 16579465
Pairs without alignments under any condition 58817192
Pairs that did not map uniquely 6487602
Genomic sequence context not extractable (edges of chromosomes) 292

Cytosine Methylation

Total C's analysed 766727977
Methylated C's in CpG context 15597730
Methylated C's in CHG context 1943098
Methylated C's in CHH context 6551324
Methylated C's in Unknown context 550110
Unmethylated C's in CpG context 113974264
Unmethylated C's in CHG context 135674473
Unmethylated C's in CHH context 492987088
Unmethylated C's in Unknown context 2355958
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3888257 original top strand
CTOT 4389764 complementary to original top strand
CTOB 4405867 complementary to original bottom strand
OB 3895285 original bottom strand

Deduplication

Alignments analysed 16579173
Unique alignments 8545472
Duplicates removed 8033701
Duplicated alignments were found at 4066044 different positions

Cytosine Methylation after Extraction

Total C's analysed 262016286
Methylated C's in CpG context 5355557
Methylated C's in CHG context 681300
Methylated C's in CHH context 2341388
Unmethylated C's in CpG context 38663960
Unmethylated C's in CHG context 46011489
Unmethylated C's in CHH context 168962592
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 1.4%

M-Bias Plot