Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth17.fastp-trim.202003063731_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth17.fastp-trim.202003063731_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 78388365 Number of paired-end alignments with a unique best hit: 19359825 Mapping efficiency: 24.7% Sequence pairs with no alignments under any condition: 50604520 Sequence pairs did not map uniquely: 8424020 Sequence pairs which were discarded because genomic sequence could not be extracted: 273 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4748276 ((converted) top strand) GA/CT/CT: 4917675 (complementary to (converted) top strand) GA/CT/GA: 4920678 (complementary to (converted) bottom strand) CT/GA/GA: 4772923 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 873966140 Total methylated C's in CpG context: 21218332 Total methylated C's in CHG context: 2381255 Total methylated C's in CHH context: 8173030 Total methylated C's in Unknown context: 786259 Total unmethylated C's in CpG context: 129235842 Total unmethylated C's in CHG context: 154077043 Total unmethylated C's in CHH context: 558880638 Total unmethylated C's in Unknown context: 2896344 C methylated in CpG context: 14.1% C methylated in CHG context: 1.5% C methylated in CHH context: 1.4% C methylated in unknown context (CN or CHN): 21.4% Bismark completed in 0d 4h 2m 20s