Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth16.fastp-trim.202003062412_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth16.fastp-trim.202003062412_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 71022035 Number of paired-end alignments with a unique best hit: 17855851 Mapping efficiency: 25.1% Sequence pairs with no alignments under any condition: 45741052 Sequence pairs did not map uniquely: 7425132 Sequence pairs which were discarded because genomic sequence could not be extracted: 353 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4399237 ((converted) top strand) GA/CT/CT: 4512869 (complementary to (converted) top strand) GA/CT/GA: 4517909 (complementary to (converted) bottom strand) CT/GA/GA: 4425483 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 831847910 Total methylated C's in CpG context: 21720278 Total methylated C's in CHG context: 2347770 Total methylated C's in CHH context: 7887418 Total methylated C's in Unknown context: 800649 Total unmethylated C's in CpG context: 118443784 Total unmethylated C's in CHG context: 145792389 Total unmethylated C's in CHH context: 535656271 Total unmethylated C's in Unknown context: 2757619 C methylated in CpG context: 15.5% C methylated in CHG context: 1.6% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 22.5% Bismark completed in 0d 3h 45m 56s