Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth15.fastp-trim.202003065503_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth15.fastp-trim.202003065503_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000000 Number of paired-end alignments with a unique best hit: 53148550 Mapping efficiency: 53.1% Sequence pairs with no alignments under any condition: 15564204 Sequence pairs did not map uniquely: 31287246 Sequence pairs which were discarded because genomic sequence could not be extracted: 367 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 12843923 ((converted) top strand) GA/CT/CT: 13723742 (complementary to (converted) top strand) GA/CT/GA: 13736475 (complementary to (converted) bottom strand) CT/GA/GA: 12844043 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2543980988 Total methylated C's in CpG context: 72241382 Total methylated C's in CHG context: 5440664 Total methylated C's in CHH context: 14488776 Total methylated C's in Unknown context: 81069 Total unmethylated C's in CpG context: 475981494 Total unmethylated C's in CHG context: 469693006 Total unmethylated C's in CHH context: 1506135666 Total unmethylated C's in Unknown context: 2701046 C methylated in CpG context: 13.2% C methylated in CHG context: 1.1% C methylated in CHH context: 1.0% C methylated in unknown context (CN or CHN): 2.9% Bismark completed in 0d 9h 11m 13s