Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth11.fastp-trim.202003065734_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth11.fastp-trim.202003065734_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 55809314 Number of paired-end alignments with a unique best hit: 1753832 Mapping efficiency: 3.1% Sequence pairs with no alignments under any condition: 53380684 Sequence pairs did not map uniquely: 674798 Sequence pairs which were discarded because genomic sequence could not be extracted: 29 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 405991 ((converted) top strand) GA/CT/CT: 470482 (complementary to (converted) top strand) GA/CT/GA: 471496 (complementary to (converted) bottom strand) CT/GA/GA: 405834 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 81765934 Total methylated C's in CpG context: 1781666 Total methylated C's in CHG context: 188076 Total methylated C's in CHH context: 646329 Total methylated C's in Unknown context: 47916 Total unmethylated C's in CpG context: 12041967 Total unmethylated C's in CHG context: 14571661 Total unmethylated C's in CHH context: 52536235 Total unmethylated C's in Unknown context: 237161 C methylated in CpG context: 12.9% C methylated in CHG context: 1.3% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 16.8% Bismark completed in 0d 0h 33m 50s