Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth10.fastp-trim.202003063900_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth10.fastp-trim.202003063900_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 81884259 Number of paired-end alignments with a unique best hit: 16579465 Mapping efficiency: 20.2% Sequence pairs with no alignments under any condition: 58817192 Sequence pairs did not map uniquely: 6487602 Sequence pairs which were discarded because genomic sequence could not be extracted: 292 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3888257 ((converted) top strand) GA/CT/CT: 4389764 (complementary to (converted) top strand) GA/CT/GA: 4405867 (complementary to (converted) bottom strand) CT/GA/GA: 3895285 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 766727977 Total methylated C's in CpG context: 15597730 Total methylated C's in CHG context: 1943098 Total methylated C's in CHH context: 6551324 Total methylated C's in Unknown context: 550110 Total unmethylated C's in CpG context: 113974264 Total unmethylated C's in CHG context: 135674473 Total unmethylated C's in CHH context: 492987088 Total unmethylated C's in Unknown context: 2355958 C methylated in CpG context: 12.0% C methylated in CHG context: 1.4% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 18.9% Bismark completed in 0d 3h 17m 2s