Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Mc/Meth14.fastp-trim.202003064415_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Mc/Meth14.fastp-trim.202003064415_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 54473 Mapping efficiency: 54.5% Sequence pairs with no alignments under any condition: 13041 Sequence pairs did not map uniquely: 32486 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 14848 ((converted) top strand) GA/CT/CT: 12378 (complementary to (converted) top strand) GA/CT/GA: 12472 (complementary to (converted) bottom strand) CT/GA/GA: 14775 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2574300 Total methylated C's in CpG context: 71703 Total methylated C's in CHG context: 3865 Total methylated C's in CHH context: 9399 Total methylated C's in Unknown context: 66 Total unmethylated C's in CpG context: 497536 Total unmethylated C's in CHG context: 482043 Total unmethylated C's in CHH context: 1509754 Total unmethylated C's in Unknown context: 2726 C methylated in CpG context: 12.6% C methylated in CHG context: 0.8% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 2.4% Bismark completed in 0d 0h 0m 44s