Bismark report for: /gscratch/scrubbed/strigg/analyses/20200306/Meth14_R1_001_val_1.fq.gz and /gscratch/scrubbed/strigg/analyses/20200306/Meth14_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 409029 Mapping efficiency: 4.1% Sequence pairs with no alignments under any condition: 9357718 Sequence pairs did not map uniquely: 233253 Sequence pairs which were discarded because genomic sequence could not be extracted: 887 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 98742 ((converted) top strand) GA/CT/CT: 114470 (complementary to (converted) top strand) GA/CT/GA: 96576 (complementary to (converted) bottom strand) CT/GA/GA: 98354 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 10076541 Total methylated C's in CpG context: 131463 Total methylated C's in CHG context: 75459 Total methylated C's in CHH context: 1102086 Total methylated C's in Unknown context: 93770 Total unmethylated C's in CpG context: 1296948 Total unmethylated C's in CHG context: 1566639 Total unmethylated C's in CHH context: 5903946 Total unmethylated C's in Unknown context: 59465 C methylated in CpG context: 9.2% C methylated in CHG context: 4.6% C methylated in CHH context: 15.7% C methylated in unknown context (CN or CHN): 61.2% Bismark completed in 0d 0h 11m 42s