Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth5.fastp-trim.202003064408_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth5.fastp-trim.202003064408_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/C1_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 4435 Mapping efficiency: 4.4% Sequence pairs with no alignments under any condition: 93364 Sequence pairs did not map uniquely: 2201 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1139 ((converted) top strand) GA/CT/CT: 1223 (complementary to (converted) top strand) GA/CT/GA: 1146 (complementary to (converted) bottom strand) CT/GA/GA: 927 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 122300 Total methylated C's in CpG context: 9909 Total methylated C's in CHG context: 580 Total methylated C's in CHH context: 16204 Total methylated C's in Unknown context: 154 Total unmethylated C's in CpG context: 13496 Total unmethylated C's in CHG context: 17302 Total unmethylated C's in CHH context: 64809 Total unmethylated C's in Unknown context: 576 C methylated in CpG context: 42.3% C methylated in CHG context: 3.2% C methylated in CHH context: 20.0% C methylated in unknown context (CN or CHN): 21.1% Bismark completed in 0d 0h 1m 19s