Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth2.fastp-trim.202003062630_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth2.fastp-trim.202003062630_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/C1_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 1696 Mapping efficiency: 1.7% Sequence pairs with no alignments under any condition: 98097 Sequence pairs did not map uniquely: 207 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 378 ((converted) top strand) GA/CT/CT: 463 (complementary to (converted) top strand) GA/CT/GA: 510 (complementary to (converted) bottom strand) CT/GA/GA: 344 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 78606 Total methylated C's in CpG context: 9028 Total methylated C's in CHG context: 524 Total methylated C's in CHH context: 3424 Total methylated C's in Unknown context: 89 Total unmethylated C's in CpG context: 1764 Total unmethylated C's in CHG context: 14663 Total unmethylated C's in CHH context: 49203 Total unmethylated C's in Unknown context: 218 C methylated in CpG context: 83.7% C methylated in CHG context: 3.5% C methylated in CHH context: 6.5% C methylated in unknown context (CN or CHN): 29.0% Bismark completed in 0d 0h 1m 19s