Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth16.fastp-trim.202003062412_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth16.fastp-trim.202003062412_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/C1_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 549 Mapping efficiency: 0.5% Sequence pairs with no alignments under any condition: 99285 Sequence pairs did not map uniquely: 166 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 129 ((converted) top strand) GA/CT/CT: 141 (complementary to (converted) top strand) GA/CT/GA: 160 (complementary to (converted) bottom strand) CT/GA/GA: 119 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 21289 Total methylated C's in CpG context: 2098 Total methylated C's in CHG context: 382 Total methylated C's in CHH context: 2485 Total methylated C's in Unknown context: 47 Total unmethylated C's in CpG context: 905 Total unmethylated C's in CHG context: 3105 Total unmethylated C's in CHH context: 12314 Total unmethylated C's in Unknown context: 100 C methylated in CpG context: 69.9% C methylated in CHG context: 11.0% C methylated in CHH context: 16.8% C methylated in unknown context (CN or CHN): 32.0% Bismark completed in 0d 0h 1m 18s