Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth15.fastp-trim.202003065503_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth15.fastp-trim.202003065503_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/C1_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 2286 Mapping efficiency: 2.3% Sequence pairs with no alignments under any condition: 95896 Sequence pairs did not map uniquely: 1818 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 503 ((converted) top strand) GA/CT/CT: 570 (complementary to (converted) top strand) GA/CT/GA: 656 (complementary to (converted) bottom strand) CT/GA/GA: 556 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 31605 Total methylated C's in CpG context: 819 Total methylated C's in CHG context: 289 Total methylated C's in CHH context: 11828 Total methylated C's in Unknown context: 147 Total unmethylated C's in CpG context: 1242 Total unmethylated C's in CHG context: 1675 Total unmethylated C's in CHH context: 15752 Total unmethylated C's in Unknown context: 291 C methylated in CpG context: 39.7% C methylated in CHG context: 14.7% C methylated in CHH context: 42.9% C methylated in unknown context (CN or CHN): 33.6% Bismark completed in 0d 0h 1m 18s