Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth12.fastp-trim.202003060645_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth12.fastp-trim.202003060645_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/C1_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 73 Mapping efficiency: 0.1% Sequence pairs with no alignments under any condition: 99904 Sequence pairs did not map uniquely: 23 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 16 ((converted) top strand) GA/CT/CT: 15 (complementary to (converted) top strand) GA/CT/GA: 23 (complementary to (converted) bottom strand) CT/GA/GA: 19 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2798 Total methylated C's in CpG context: 61 Total methylated C's in CHG context: 65 Total methylated C's in CHH context: 773 Total methylated C's in Unknown context: 18 Total unmethylated C's in CpG context: 118 Total unmethylated C's in CHG context: 151 Total unmethylated C's in CHH context: 1630 Total unmethylated C's in Unknown context: 31 C methylated in CpG context: 34.1% C methylated in CHG context: 30.1% C methylated in CHH context: 32.2% C methylated in unknown context (CN or CHN): 36.7% Bismark completed in 0d 0h 1m 19s