Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth1.fastp-trim.202003061245_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth1.fastp-trim.202003061245_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/C1_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 1159 Mapping efficiency: 1.2% Sequence pairs with no alignments under any condition: 98699 Sequence pairs did not map uniquely: 142 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 252 ((converted) top strand) GA/CT/CT: 341 (complementary to (converted) top strand) GA/CT/GA: 305 (complementary to (converted) bottom strand) CT/GA/GA: 261 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 54702 Total methylated C's in CpG context: 6128 Total methylated C's in CHG context: 316 Total methylated C's in CHH context: 2521 Total methylated C's in Unknown context: 43 Total unmethylated C's in CpG context: 1570 Total unmethylated C's in CHG context: 10277 Total unmethylated C's in CHH context: 33890 Total unmethylated C's in Unknown context: 169 C methylated in CpG context: 79.6% C methylated in CHG context: 3.0% C methylated in CHH context: 6.9% C methylated in unknown context (CN or CHN): 20.3% Bismark completed in 0d 0h 1m 21s