Bismark report for: /gscratch/srlab/sr320/data/cg/zr3534_9_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_9_R2.fastp-trim.20201202.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 73776588 Number of paired-end alignments with a unique best hit: 45563698 Mapping efficiency: 61.8% Sequence pairs with no alignments under any condition: 16033397 Sequence pairs did not map uniquely: 12179493 Sequence pairs which were discarded because genomic sequence could not be extracted: 83 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11353988 ((converted) top strand) GA/CT/CT: 11374551 (complementary to (converted) top strand) GA/CT/GA: 11426794 (complementary to (converted) bottom strand) CT/GA/GA: 11408282 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1594610721 Total methylated C's in CpG context: 21006439 Total methylated C's in CHG context: 1277410 Total methylated C's in CHH context: 5348307 Total methylated C's in Unknown context: 49314 Total unmethylated C's in CpG context: 188753220 Total unmethylated C's in CHG context: 280865922 Total unmethylated C's in CHH context: 1097359423 Total unmethylated C's in Unknown context: 3841284 C methylated in CpG context: 10.0% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 6h 30m 29s