Bismark report for: /gscratch/srlab/sr320/data/cg/zr3534_7_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_7_R2.fastp-trim.20201202.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 66080050 Number of paired-end alignments with a unique best hit: 40344190 Mapping efficiency: 61.1% Sequence pairs with no alignments under any condition: 15262949 Sequence pairs did not map uniquely: 10472911 Sequence pairs which were discarded because genomic sequence could not be extracted: 80 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10070937 ((converted) top strand) GA/CT/CT: 10071344 (complementary to (converted) top strand) GA/CT/GA: 10098220 (complementary to (converted) bottom strand) CT/GA/GA: 10103609 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1402472946 Total methylated C's in CpG context: 18233140 Total methylated C's in CHG context: 1193897 Total methylated C's in CHH context: 5124940 Total methylated C's in Unknown context: 46385 Total unmethylated C's in CpG context: 161568022 Total unmethylated C's in CHG context: 242812064 Total unmethylated C's in CHH context: 973540883 Total unmethylated C's in Unknown context: 3481104 C methylated in CpG context: 10.1% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 5h 51m 0s