Bismark report for: /gscratch/srlab/sr320/data/cg/zr3534_2_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_2_R2.fastp-trim.20201202.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 75413899 Number of paired-end alignments with a unique best hit: 46120606 Mapping efficiency: 61.2% Sequence pairs with no alignments under any condition: 17038192 Sequence pairs did not map uniquely: 12255101 Sequence pairs which were discarded because genomic sequence could not be extracted: 82 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11474842 ((converted) top strand) GA/CT/CT: 11533477 (complementary to (converted) top strand) GA/CT/GA: 11580272 (complementary to (converted) bottom strand) CT/GA/GA: 11531933 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1611369360 Total methylated C's in CpG context: 21945550 Total methylated C's in CHG context: 1369962 Total methylated C's in CHH context: 5816386 Total methylated C's in Unknown context: 53658 Total unmethylated C's in CpG context: 189972859 Total unmethylated C's in CHG context: 281749160 Total unmethylated C's in CHH context: 1110515443 Total unmethylated C's in Unknown context: 3919064 C methylated in CpG context: 10.4% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.4% Bismark completed in 0d 6h 37m 36s