Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_7_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_7_R2.fastp-trim.20201202.fq.gz

Data processed at 09:17 on 2021-03-03


Alignment Stats

Sequence pairs analysed in total 66080050
Paired-end alignments with a unique best hit 40864254
Pairs without alignments under any condition 17443984
Pairs that did not map uniquely 7771812
Genomic sequence context not extractable (edges of chromosomes) 4618

Cytosine Methylation

Total C's analysed 1419038224
Methylated C's in CpG context 18570895
Methylated C's in CHG context 1217349
Methylated C's in CHH context 5287128
Methylated C's in Unknown context 46169
Unmethylated C's in CpG context 161989672
Unmethylated C's in CHG context 246538725
Unmethylated C's in CHH context 985434455
Unmethylated C's in Unknown context 3583396
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 10223885 original top strand
CTOT 10220392 complementary to original top strand
CTOB 10209054 complementary to original bottom strand
OB 10206305 original bottom strand

Deduplication

Alignments analysed 40859636
Unique alignments 33973996
Duplicates removed 6885640
Duplicated alignments were found at 5802803 different positions

Cytosine Methylation after Extraction

Total C's analysed 817176174
Methylated C's in CpG context 10367757
Methylated C's in CHG context 689165
Methylated C's in CHH context 3016470
Unmethylated C's in CpG context 92626062
Unmethylated C's in CHG context 140442001
Unmethylated C's in CHH context 570034719
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot