Bismark report for: /gscratch/srlab/sr320/data/cg/zr3534_9_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_9_R2.fastp-trim.20201202.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 73776588 Number of paired-end alignments with a unique best hit: 46249576 Mapping efficiency: 62.7% Sequence pairs with no alignments under any condition: 18427528 Sequence pairs did not map uniquely: 9099484 Sequence pairs which were discarded because genomic sequence could not be extracted: 5273 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11561829 ((converted) top strand) GA/CT/CT: 11578586 (complementary to (converted) top strand) GA/CT/GA: 11565150 (complementary to (converted) bottom strand) CT/GA/GA: 11538738 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1614930893 Total methylated C's in CpG context: 21402276 Total methylated C's in CHG context: 1304086 Total methylated C's in CHH context: 5550413 Total methylated C's in Unknown context: 49721 Total unmethylated C's in CpG context: 188596358 Total unmethylated C's in CHG context: 285271601 Total unmethylated C's in CHH context: 1112806159 Total unmethylated C's in Unknown context: 3962829 C methylated in CpG context: 10.2% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.2% Bismark completed in 0d 7h 12m 49s