Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_6_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_6_R2.fastp-trim.20201202.fq.gz

Data processed at 08:32 on 2021-03-03


Alignment Stats

Sequence pairs analysed in total 75321697
Paired-end alignments with a unique best hit 46100544
Pairs without alignments under any condition 20284411
Pairs that did not map uniquely 8936742
Genomic sequence context not extractable (edges of chromosomes) 5798

Cytosine Methylation

Total C's analysed 1608577636
Methylated C's in CpG context 21797457
Methylated C's in CHG context 1380274
Methylated C's in CHH context 5994483
Methylated C's in Unknown context 55865
Unmethylated C's in CpG context 184014825
Unmethylated C's in CHG context 279745904
Unmethylated C's in CHH context 1115644693
Unmethylated C's in Unknown context 4091675
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11519621 original top strand
CTOT 11534009 complementary to original top strand
CTOB 11527937 complementary to original bottom strand
OB 11513179 original bottom strand

Deduplication

Alignments analysed 46094746
Unique alignments 37585028
Duplicates removed 8509718
Duplicated alignments were found at 7037435 different positions

Cytosine Methylation after Extraction

Total C's analysed 907305363
Methylated C's in CpG context 11944399
Methylated C's in CHG context 766919
Methylated C's in CHH context 3343495
Unmethylated C's in CpG context 102977222
Unmethylated C's in CHG context 156115395
Unmethylated C's in CHH context 632157933
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot