Bismark report for: /gscratch/srlab/sr320/data/cg/zr3534_1_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_1_R2.fastp-trim.20201202.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 76522716 Number of paired-end alignments with a unique best hit: 47699485 Mapping efficiency: 62.3% Sequence pairs with no alignments under any condition: 19741361 Sequence pairs did not map uniquely: 9081870 Sequence pairs which were discarded because genomic sequence could not be extracted: 5082 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11928000 ((converted) top strand) GA/CT/CT: 11941655 (complementary to (converted) top strand) GA/CT/GA: 11927676 (complementary to (converted) bottom strand) CT/GA/GA: 11897072 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1653706228 Total methylated C's in CpG context: 22660816 Total methylated C's in CHG context: 1421301 Total methylated C's in CHH context: 6086761 Total methylated C's in Unknown context: 53967 Total unmethylated C's in CpG context: 191604352 Total unmethylated C's in CHG context: 289632516 Total unmethylated C's in CHH context: 1142300482 Total unmethylated C's in Unknown context: 4118533 C methylated in CpG context: 10.6% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 7h 28m 48s