Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_1_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_1_R2.fastp-trim.20201202.fq.gz

Data processed at 08:32 on 2021-03-03


Alignment Stats

Sequence pairs analysed in total 76522716
Paired-end alignments with a unique best hit 47699485
Pairs without alignments under any condition 19741361
Pairs that did not map uniquely 9081870
Genomic sequence context not extractable (edges of chromosomes) 5082

Cytosine Methylation

Total C's analysed 1653706228
Methylated C's in CpG context 22660816
Methylated C's in CHG context 1421301
Methylated C's in CHH context 6086761
Methylated C's in Unknown context 53967
Unmethylated C's in CpG context 191604352
Unmethylated C's in CHG context 289632516
Unmethylated C's in CHH context 1142300482
Unmethylated C's in Unknown context 4118533
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11928000 original top strand
CTOT 11941655 complementary to original top strand
CTOB 11927676 complementary to original bottom strand
OB 11897072 original bottom strand

Deduplication

Alignments analysed 47694403
Unique alignments 38704882
Duplicates removed 8989521
Duplicated alignments were found at 7455505 different positions

Cytosine Methylation after Extraction

Total C's analysed 925374274
Methylated C's in CpG context 12261150
Methylated C's in CHG context 780318
Methylated C's in CHH context 3368890
Unmethylated C's in CpG context 106316812
Unmethylated C's in CHG context 160195807
Unmethylated C's in CHH context 642451297
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot