Bismark report for: /gscratch/srlab/sr320/data/cg/zr3644_22_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_22_R2.fastp-trim.20201206.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 46739729 Number of paired-end alignments with a unique best hit: 18025102 Mapping efficiency: 38.6% Sequence pairs with no alignments under any condition: 24265313 Sequence pairs did not map uniquely: 4449314 Sequence pairs which were discarded because genomic sequence could not be extracted: 746 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4499080 ((converted) top strand) GA/CT/CT: 4503722 (complementary to (converted) top strand) GA/CT/GA: 4511659 (complementary to (converted) bottom strand) CT/GA/GA: 4509895 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 644615809 Total methylated C's in CpG context: 10062264 Total methylated C's in CHG context: 520139 Total methylated C's in CHH context: 2039584 Total methylated C's in Unknown context: 4491 Total unmethylated C's in CpG context: 73624566 Total unmethylated C's in CHG context: 114700996 Total unmethylated C's in CHH context: 443668260 Total unmethylated C's in Unknown context: 443014 C methylated in CpG context: 12.0% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.0% Bismark completed in 0d 6h 13m 37s