6 -I{} /gscratch/srlab/programs/Bismark-0.21.0/bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ -p 4 --non_directional -1 /gscratch/srlab/sr320/data/cg/{}_R1.fastp-trim.20201206.fq.gz -2 /gscratch/srlab/sr320/data/cg/{}_R2.fastp-trim.20201206.fq.gz zr3644_10 zr3644_11 zr3644_12 zr3644_13 zr3644_14 zr3644_15 zr3644_16 zr3644_17 zr3644_18 zr3644_19 zr3644_1 zr3644_20 zr3644_21 zr3644_22 zr3644_23 zr3644_24 zr3644_2 zr3644_3 zr3644_4 zr3644_5 zr3644_6 zr3644_7 zr3644_8 zr3644_9 xargs: failed to open /dev/tty for reading: No such device or address find: ‘*.bam’: No such file or directory basename: missing operand Try 'basename --help' for more information. *** Bismark methylation extractor version v0.21.0 *** Trying to determine the type of mapping from the SAM header line of file *deduplicated.bam [FATAL ERROR]: File >>*deduplicated.bam<< did not exist. Please re-specify file names and try again... Found no potential alignment reports in the current directory. Please specify a single Bismark alignment report file using the option '--alignment_report FILE' SYNOPSIS: This script uses a Bismark alignment report to generate a graphical HTML report page. Optionally, further reports of the Bismark suite such as deduplication, methylation extractor splitting or M-bias reports can be specified as well. If several Bismark reports are found in the same folder, a separate report will be generated for each of these, whereby the output filename will be derived from the Bismark alignment report file. bismark2report attempts to find optional reports automatically based on the file basename. USAGE: bismark2report [options] -o/--output Name of the output file (optional). If not specified explicitly, the output filename will be derived from the Bismark alignment report file. Specifying an output filename only works if the HTML report is to be generated for a single Bismark alignment report (and potentially additional reports). --dir Output directory. Output is written to the current directory if not specified explicitly. --alignment_report FILE If not specified explicitly, bismark2report attempts to find Bismark report file(s) in the current directory and produces a separate HTML report for each mapping report file. Based on the basename of the Bismark mapping report, bismark2report will also attempt to find the other Bismark reports (see below) for inclusion into the HTML report. Specifying a Bismark alignment report file is mandatory. --dedup_report FILE If not specified explicitly, bismark2report attempts to find a deduplication report file with the same basename as the Bismark mapping report (generated by deduplicate_bismark) in the current working directory. Including a deduplication report is optional, and using the FILE 'none' will skip this step entirely. --splitting_report FILE If not specified explicitly, bismark2report attempts to find a splitting report file with the same basename as the Bismark mapping report (generated by the Bismark methylation extractor) in the current working directory. Including a splitting report is optional, and using the FILE 'none' will skip this step entirely. --mbias_report FILE If not specified explicitly, bismark2report attempts to find a single M-bias report file with the same basename as the Bismark mapping report (generated by the Bismark methylation extractor) in the current working directory. Including an M-Bias report is optional, and using the FILE 'none' will skip this step entirely. --nucleotide_report FILE If not specified explicitly, bismark2report attempts to find a single nucleotide coverage report file with the same basename as the Bismark mapping report (generated by Bismark with the option '--nucleotide_coverage') in the current working directory. Including a nucleotide coverage statistics report is optional, and using the FILE 'none' will skip this report entirely. Script last modified: 07 August 2018 No Bismark/Bowtie2 single-end BAM files detected No Bismark/Bowtie2 paired-end BAM files detected No Bismark/HISAT2 single-end BAM files detected No Bismark/HISAT2 paired-end BAM files detected Error: No Bismark BAM files found to generate a Bismark project summary. Please respecify... USAGE: bismark2summary (*.bam), or bismark2summary --help for more information at /gscratch/srlab/programs/Bismark-0.21.0/bismark2summary line 201. find: ‘*deduplicated.bam’: No such file or directory basename: missing operand Try 'basename --help' for more information. find: ‘*.sorted.bam’: No such file or directory basename: missing operand Try 'basename --help' for more information.