Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_8_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_8_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 42084665
Paired-end alignments with a unique best hit 15895747
Pairs without alignments under any condition 22183581
Pairs that did not map uniquely 4005337
Genomic sequence context not extractable (edges of chromosomes) 652

Cytosine Methylation

Total C's analysed 563563445
Methylated C's in CpG context 8959468
Methylated C's in CHG context 452821
Methylated C's in CHH context 1795696
Methylated C's in Unknown context 3900
Unmethylated C's in CpG context 63771689
Unmethylated C's in CHG context 99628661
Unmethylated C's in CHH context 388955110
Unmethylated C's in Unknown context 376750
Percentage methylation (CpG context) 12.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3991732 original top strand
CTOT 3949846 complementary to original top strand
CTOB 3953198 complementary to original bottom strand
OB 4000319 original bottom strand

Deduplication

Alignments analysed 15895095
Unique alignments 14242845
Duplicates removed 1652250
Duplicated alignments were found at 1478899 different positions

Cytosine Methylation after Extraction

Total C's analysed 336126373
Methylated C's in CpG context 5237785
Methylated C's in CHG context 267711
Methylated C's in CHH context 1067626
Unmethylated C's in CpG context 37832107
Unmethylated C's in CHG context 58950807
Unmethylated C's in CHH context 232770337
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot