Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_7_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_7_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 81002273
Paired-end alignments with a unique best hit 30152005
Pairs without alignments under any condition 43523098
Pairs that did not map uniquely 7327170
Genomic sequence context not extractable (edges of chromosomes) 1319

Cytosine Methylation

Total C's analysed 1073858102
Methylated C's in CpG context 16768704
Methylated C's in CHG context 841729
Methylated C's in CHH context 3357119
Methylated C's in Unknown context 7582
Unmethylated C's in CpG context 120673829
Unmethylated C's in CHG context 188146912
Unmethylated C's in CHH context 744069809
Unmethylated C's in Unknown context 752422
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7566344 original top strand
CTOT 7498585 complementary to original top strand
CTOB 7510631 complementary to original bottom strand
OB 7575126 original bottom strand

Deduplication

Alignments analysed 30150686
Unique alignments 26850541
Duplicates removed 3300145
Duplicated alignments were found at 2923521 different positions

Cytosine Methylation after Extraction

Total C's analysed 644300662
Methylated C's in CpG context 9851900
Methylated C's in CHG context 499230
Methylated C's in CHH context 2005314
Unmethylated C's in CpG context 71957703
Unmethylated C's in CHG context 111910013
Unmethylated C's in CHH context 448076502
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot