Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_6_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_6_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 52883485
Paired-end alignments with a unique best hit 20160317
Pairs without alignments under any condition 27836055
Pairs that did not map uniquely 4887113
Genomic sequence context not extractable (edges of chromosomes) 807
38.1%52.6%9.24%0.00153%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 718298612
Methylated C's in CpG context 10965060
Methylated C's in CHG context 562730
Methylated C's in CHH context 2250413
Methylated C's in Unknown context 4995
Unmethylated C's in CpG context 81037261
Unmethylated C's in CHG context 126317382
Unmethylated C's in CHH context 497165766
Unmethylated C's in Unknown context 501305
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 5066119 original top strand
CTOT 5015207 complementary to original top strand
CTOB 5015651 complementary to original bottom strand
OB 5062533 original bottom strand
OTCTOTCTOBOB01M2M3M4M5M
Number of Alignments

Deduplication

Alignments analysed 20159510
Unique alignments 17804674
Duplicates removed 2354836
Duplicated alignments were found at 2080181 different positions
88.3%11.7%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 424685961
Methylated C's in CpG context 6360295
Methylated C's in CHG context 329265
Methylated C's in CHH context 1324855
Unmethylated C's in CpG context 47688586
Unmethylated C's in CHG context 74108907
Unmethylated C's in CHH context 294874053
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

204060801000204060801000.5M1M1.5M2M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls

2040608010002040608010000.2M0.4M0.6M0.8M1M1.2M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 2Position in Read [bp]% Methylation# Methylation Calls