Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_5_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_5_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 43865548
Paired-end alignments with a unique best hit 16134171
Pairs without alignments under any condition 23833705
Pairs that did not map uniquely 3897672
Genomic sequence context not extractable (edges of chromosomes) 631
36.8%54.3%8.89%0.00144%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 575564351
Methylated C's in CpG context 8906914
Methylated C's in CHG context 449265
Methylated C's in CHH context 1780161
Methylated C's in Unknown context 3885
Unmethylated C's in CpG context 64701267
Unmethylated C's in CHG context 101216510
Unmethylated C's in CHH context 398510234
Unmethylated C's in Unknown context 390437
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 4010854 original top strand
CTOT 4046584 complementary to original top strand
CTOB 4055514 complementary to original bottom strand
OB 4020588 original bottom strand
OTCTOTCTOBOB00.5M1M1.5M2M2.5M3M3.5M4M
Number of Alignments

Deduplication

Alignments analysed 16133540
Unique alignments 14477780
Duplicates removed 1655760
Duplicated alignments were found at 1482966 different positions
89.7%10.3%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 347116867
Methylated C's in CpG context 5264304
Methylated C's in CHG context 268683
Methylated C's in CHH context 1069376
Unmethylated C's in CpG context 38821341
Unmethylated C's in CHG context 60538663
Unmethylated C's in CHH context 241154500
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

204060801000204060801000.2M0.4M0.6M0.8M1M1.2M1.4M1.6M1.8M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls

2040608010002040608010000.2M0.4M0.6M0.8M1M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 2Position in Read [bp]% Methylation# Methylation Calls