Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_5_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_5_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 43865548
Paired-end alignments with a unique best hit 16134171
Pairs without alignments under any condition 23833705
Pairs that did not map uniquely 3897672
Genomic sequence context not extractable (edges of chromosomes) 631

Cytosine Methylation

Total C's analysed 575564351
Methylated C's in CpG context 8906914
Methylated C's in CHG context 449265
Methylated C's in CHH context 1780161
Methylated C's in Unknown context 3885
Unmethylated C's in CpG context 64701267
Unmethylated C's in CHG context 101216510
Unmethylated C's in CHH context 398510234
Unmethylated C's in Unknown context 390437
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4010854 original top strand
CTOT 4046584 complementary to original top strand
CTOB 4055514 complementary to original bottom strand
OB 4020588 original bottom strand

Deduplication

Alignments analysed 16133540
Unique alignments 14477780
Duplicates removed 1655760
Duplicated alignments were found at 1482966 different positions

Cytosine Methylation after Extraction

Total C's analysed 347116867
Methylated C's in CpG context 5264304
Methylated C's in CHG context 268683
Methylated C's in CHH context 1069376
Unmethylated C's in CpG context 38821341
Unmethylated C's in CHG context 60538663
Unmethylated C's in CHH context 241154500
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot