Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_4_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_4_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 49072984
Paired-end alignments with a unique best hit 18288301
Pairs without alignments under any condition 26298409
Pairs that did not map uniquely 4486274
Genomic sequence context not extractable (edges of chromosomes) 669

Cytosine Methylation

Total C's analysed 651332942
Methylated C's in CpG context 9707910
Methylated C's in CHG context 484408
Methylated C's in CHH context 1922996
Methylated C's in Unknown context 4291
Unmethylated C's in CpG context 73601851
Unmethylated C's in CHG context 113783118
Unmethylated C's in CHH context 451832659
Unmethylated C's in Unknown context 448038
Percentage methylation (CpG context) 11.7%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4594086 original top strand
CTOT 4547569 complementary to original top strand
CTOB 4551018 complementary to original bottom strand
OB 4594959 original bottom strand

Deduplication

Alignments analysed 18287632
Unique alignments 16239837
Duplicates removed 2047795
Duplicated alignments were found at 1825523 different positions

Cytosine Methylation after Extraction

Total C's analysed 385896195
Methylated C's in CpG context 5636835
Methylated C's in CHG context 284080
Methylated C's in CHH context 1138667
Unmethylated C's in CpG context 43385065
Unmethylated C's in CHG context 66885343
Unmethylated C's in CHH context 268566205
Percentage methylation (CpG context) 11.5%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot