Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_2_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_2_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 54652876
Paired-end alignments with a unique best hit 20369855
Pairs without alignments under any condition 29274584
Pairs that did not map uniquely 5008437
Genomic sequence context not extractable (edges of chromosomes) 871

Cytosine Methylation

Total C's analysed 732580403
Methylated C's in CpG context 11051885
Methylated C's in CHG context 565587
Methylated C's in CHH context 2253641
Methylated C's in Unknown context 5147
Unmethylated C's in CpG context 82749931
Unmethylated C's in CHG context 128234690
Unmethylated C's in CHH context 507724669
Unmethylated C's in Unknown context 520438
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5114698 original top strand
CTOT 5071756 complementary to original top strand
CTOB 5076555 complementary to original bottom strand
OB 5105975 original bottom strand

Deduplication

Alignments analysed 20368984
Unique alignments 17900177
Duplicates removed 2468807
Duplicated alignments were found at 2167026 different positions

Cytosine Methylation after Extraction

Total C's analysed 440995801
Methylated C's in CpG context 6524098
Methylated C's in CHG context 336393
Methylated C's in CHH context 1347203
Unmethylated C's in CpG context 49502508
Unmethylated C's in CHG context 76570408
Unmethylated C's in CHH context 306715191
Percentage methylation (CpG context) 11.6%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot