Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_24_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_24_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 51969546
Paired-end alignments with a unique best hit 20006798
Pairs without alignments under any condition 27019300
Pairs that did not map uniquely 4943448
Genomic sequence context not extractable (edges of chromosomes) 858

Cytosine Methylation

Total C's analysed 715252030
Methylated C's in CpG context 11050092
Methylated C's in CHG context 570230
Methylated C's in CHH context 2241430
Methylated C's in Unknown context 4893
Unmethylated C's in CpG context 81415730
Unmethylated C's in CHG context 126881295
Unmethylated C's in CHH context 493093253
Unmethylated C's in Unknown context 493097
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5025646 original top strand
CTOT 4972004 complementary to original top strand
CTOB 4978007 complementary to original bottom strand
OB 5030283 original bottom strand

Deduplication

Alignments analysed 20005940
Unique alignments 17708602
Duplicates removed 2297338
Duplicated alignments were found at 2034991 different positions

Cytosine Methylation after Extraction

Total C's analysed 424334642
Methylated C's in CpG context 6426886
Methylated C's in CHG context 334748
Methylated C's in CHH context 1323628
Unmethylated C's in CpG context 48012879
Unmethylated C's in CHG context 74644900
Unmethylated C's in CHH context 293591601
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot