Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_23_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_23_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 39675229
Paired-end alignments with a unique best hit 15280432
Pairs without alignments under any condition 20537283
Pairs that did not map uniquely 3857514
Genomic sequence context not extractable (edges of chromosomes) 685

Cytosine Methylation

Total C's analysed 541472367
Methylated C's in CpG context 8318708
Methylated C's in CHG context 431809
Methylated C's in CHH context 1705670
Methylated C's in Unknown context 3697
Unmethylated C's in CpG context 61834458
Unmethylated C's in CHG context 96009612
Unmethylated C's in CHH context 373172110
Unmethylated C's in Unknown context 370042
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3852890 original top strand
CTOT 3784475 complementary to original top strand
CTOB 3787300 complementary to original bottom strand
OB 3855082 original bottom strand

Deduplication

Alignments analysed 15279747
Unique alignments 13580699
Duplicates removed 1699048
Duplicated alignments were found at 1517509 different positions

Cytosine Methylation after Extraction

Total C's analysed 319217422
Methylated C's in CpG context 4796647
Methylated C's in CHG context 251840
Methylated C's in CHH context 1001795
Unmethylated C's in CpG context 36244726
Unmethylated C's in CHG context 56117649
Unmethylated C's in CHH context 220804765
Percentage methylation (CpG context) 11.7%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%

M-Bias Plot