Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_22_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_22_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 46739729
Paired-end alignments with a unique best hit 18025102
Pairs without alignments under any condition 24265313
Pairs that did not map uniquely 4449314
Genomic sequence context not extractable (edges of chromosomes) 746

Cytosine Methylation

Total C's analysed 644615809
Methylated C's in CpG context 10062264
Methylated C's in CHG context 520139
Methylated C's in CHH context 2039584
Methylated C's in Unknown context 4491
Unmethylated C's in CpG context 73624566
Unmethylated C's in CHG context 114700996
Unmethylated C's in CHH context 443668260
Unmethylated C's in Unknown context 443014
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4499080 original top strand
CTOT 4503722 complementary to original top strand
CTOB 4511659 complementary to original bottom strand
OB 4509895 original bottom strand

Deduplication

Alignments analysed 18024356
Unique alignments 16001443
Duplicates removed 2022913
Duplicated alignments were found at 1800814 different positions

Cytosine Methylation after Extraction

Total C's analysed 384123373
Methylated C's in CpG context 5860168
Methylated C's in CHG context 306457
Methylated C's in CHH context 1207901
Unmethylated C's in CpG context 43577975
Unmethylated C's in CHG context 67741077
Unmethylated C's in CHH context 265429795
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot